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goat antimouse vcam  (R&D Systems)


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    Structured Review

    R&D Systems goat antimouse vcam
    Goat Antimouse Vcam, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+antimouse+vcam/Mouse+VCAM-1%2FCD106+Antibody/pmc12883212-68-55-59
    Average 93 stars, based on 83 article reviews
    goat antimouse vcam - by Bioz Stars, 2026-10
    93/100 stars

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    Incubation:

    Article Title: The influence of VEGFR-2 blockade and focused ultrasound blood–brain barrier opening on the glioma-immune landscape
    Article Snippet: .. Sections were then incubated overnight at 4°C with a varied combination of Alexa Fluor 488 antimouse CD31 (1:200, BioLegend #102514), Alex Fluor594 antimouse CD31 antibody (1:200, BioLegend #102520), APC/Fire 750 antimouse CD45 (1:750, BioLegend #103154), Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6) (1:400, ThermoFisher #MA5-14520), chicken anti-GFAP (1:5000, ThermoFisher #PA1-10004), rabbit anti-ICAM (1:500, ThermoFisher #10020-1-AP), and goat antimouse VCAM-1/CD106 (1:1000, R&D Systems #AF643). .. After washing the slides 3x for 10 min in 0.1% Tween-20 PBS, sections were incubated for 1 hr at room temp with a combination of donkey anti-rabbit Alexa Fluor 647 (1:800, ThermoFisher #A-31573), donkey antichicken Alexa Fluor 488 (1:2000, ThermoFisher #A78948), donkey antirabbit Alexa Fluor 568 (1:1000, ThermoFisher #A10042), and donkey antigoat Alexa Fluor 647 (1:1000, ThermoFisher #A-21447).

    Article Title: VCAM-1-mediated neutrophil infiltration exacerbates ambient fine particle-induced lung injury.
    Article Snippet: Background: Fine ambient particle matter (PM2.5) induces inflammatory lung injury; however, whether intratracheal administration of PM2.5 increases pulmonary polymorphonuclear leukocyte (PMN) infiltration, the mechanism of infiltration, and if these cells exacerbate PM2.5induced lung injury are unknown.. Methods: Using 32,704 subjects, the association between blood PMNs and ambient PM2.5 levels on the previous day was retrospectively analyzed.. Neutropenia was achieved by injecting mice with PMN-specific antibodies.

    Recombinant:

    Article Title: The influence of VEGFR-2 blockade and focused ultrasound blood–brain barrier opening on the glioma-immune landscape
    Article Snippet: .. Sections were then incubated overnight at 4°C with a varied combination of Alexa Fluor 488 antimouse CD31 (1:200, BioLegend #102514), Alex Fluor594 antimouse CD31 antibody (1:200, BioLegend #102520), APC/Fire 750 antimouse CD45 (1:750, BioLegend #103154), Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6) (1:400, ThermoFisher #MA5-14520), chicken anti-GFAP (1:5000, ThermoFisher #PA1-10004), rabbit anti-ICAM (1:500, ThermoFisher #10020-1-AP), and goat antimouse VCAM-1/CD106 (1:1000, R&D Systems #AF643). .. After washing the slides 3x for 10 min in 0.1% Tween-20 PBS, sections were incubated for 1 hr at room temp with a combination of donkey anti-rabbit Alexa Fluor 647 (1:800, ThermoFisher #A-31573), donkey antichicken Alexa Fluor 488 (1:2000, ThermoFisher #A78948), donkey antirabbit Alexa Fluor 568 (1:1000, ThermoFisher #A10042), and donkey antigoat Alexa Fluor 647 (1:1000, ThermoFisher #A-21447).

    Blocking Assay:

    Article Title: VCAM-1-mediated neutrophil infiltration exacerbates ambient fine particle-induced lung injury.
    Article Snippet: Background: Fine ambient particle matter (PM2.5) induces inflammatory lung injury; however, whether intratracheal administration of PM2.5 increases pulmonary polymorphonuclear leukocyte (PMN) infiltration, the mechanism of infiltration, and if these cells exacerbate PM2.5induced lung injury are unknown.. Methods: Using 32,704 subjects, the association between blood PMNs and ambient PM2.5 levels on the previous day was retrospectively analyzed.. Neutropenia was achieved by injecting mice with PMN-specific antibodies.

    Activity Assay:

    Article Title: VCAM-1-mediated neutrophil infiltration exacerbates ambient fine particle-induced lung injury.
    Article Snippet: Background: Fine ambient particle matter (PM2.5) induces inflammatory lung injury; however, whether intratracheal administration of PM2.5 increases pulmonary polymorphonuclear leukocyte (PMN) infiltration, the mechanism of infiltration, and if these cells exacerbate PM2.5induced lung injury are unknown.. Methods: Using 32,704 subjects, the association between blood PMNs and ambient PM2.5 levels on the previous day was retrospectively analyzed.. Neutropenia was achieved by injecting mice with PMN-specific antibodies.



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    <t>VCAM‐1</t> <t>is</t> up‐regulated in tumor‐associated LECs in 4T1 tumors deficient of VCAM‐1. ( a ) Schematic representation of the strategy to delete VCAM‐1 in 4T1 tumor cells. Two neighboring PAM motifs on the top and the bottom strand were identified within exon 3 of the vcam1 gene (triangles). Guide‐RNAs for adjacent target sequences (in italics) together with the Cas9n enzyme were transfected into 4T1 tumor cells and single knockout clones were isolated by FACS sorting. ( b ) Representative FACS histogram of VCAM‐1 staining using a polyclonal antibody in a control clone (XF9) transfected with Cas9n only and a complete VCAM‐1 knockout clone (1F8). ( c ) 4T1‐1F8 cells were implanted into Balb/c mice and the expression of VCAM‐1 on tumor‐associated LECs was determined by FACS on Day 21 after implantation ( n = 5 mice/group; * p < 0.05).
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    Image Search Results


    VCAM‐1 is up‐regulated in tumor‐associated LECs in 4T1 tumors deficient of VCAM‐1. ( a ) Schematic representation of the strategy to delete VCAM‐1 in 4T1 tumor cells. Two neighboring PAM motifs on the top and the bottom strand were identified within exon 3 of the vcam1 gene (triangles). Guide‐RNAs for adjacent target sequences (in italics) together with the Cas9n enzyme were transfected into 4T1 tumor cells and single knockout clones were isolated by FACS sorting. ( b ) Representative FACS histogram of VCAM‐1 staining using a polyclonal antibody in a control clone (XF9) transfected with Cas9n only and a complete VCAM‐1 knockout clone (1F8). ( c ) 4T1‐1F8 cells were implanted into Balb/c mice and the expression of VCAM‐1 on tumor‐associated LECs was determined by FACS on Day 21 after implantation ( n = 5 mice/group; * p < 0.05).

    Journal: International Journal of Cancer

    Article Title: Transcriptional profiling of breast cancer‐associated lymphatic vessels reveals VCAM‐1 as regulator of lymphatic invasion and permeability

    doi: 10.1002/ijc.32594

    Figure Lengend Snippet: VCAM‐1 is up‐regulated in tumor‐associated LECs in 4T1 tumors deficient of VCAM‐1. ( a ) Schematic representation of the strategy to delete VCAM‐1 in 4T1 tumor cells. Two neighboring PAM motifs on the top and the bottom strand were identified within exon 3 of the vcam1 gene (triangles). Guide‐RNAs for adjacent target sequences (in italics) together with the Cas9n enzyme were transfected into 4T1 tumor cells and single knockout clones were isolated by FACS sorting. ( b ) Representative FACS histogram of VCAM‐1 staining using a polyclonal antibody in a control clone (XF9) transfected with Cas9n only and a complete VCAM‐1 knockout clone (1F8). ( c ) 4T1‐1F8 cells were implanted into Balb/c mice and the expression of VCAM‐1 on tumor‐associated LECs was determined by FACS on Day 21 after implantation ( n = 5 mice/group; * p < 0.05).

    Article Snippet: For staining of cultured cells, cells were harvested using trypsin and subsequently stained with Itga4‐FITC (Biolegend 103606), Itgb1‐FITC (Biolegend 102206), goat antimouse Itga9 (R&D, Minneapolis, MN, AF3827) or goat antimouse VCAM‐1 (R&D AF643), followed by a donkey antigoat‐Alexa488 antibody.

    Techniques: Transfection, Knock-Out, Clone Assay, Isolation, Staining, Control, Expressing